Brown Algae Fucoxanthin Is Hydrolyzed to Fucoxanthinol during Absorption by Caco-2 Human Intestinal Cells and Mice

Tatsuya, Sugawara and Vallikannan, Baskaran and Wakako, Tsuzuki and Akihiko, Nagao (2002) Brown Algae Fucoxanthin Is Hydrolyzed to Fucoxanthinol during Absorption by Caco-2 Human Intestinal Cells and Mice. The Journal of Nutrition, 132 (5). pp. 946-951.

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Abstract

The metabolic fate in mammals of dietary fucoxanthin, a major carotenoid in brown algae, is not known. We investigated the absorption and metabolism of fucoxanthin in differentiated Caco-2 human intestinal cells, a useful model for studying the absorption of dietary compounds by intestinal cells. Fucoxanthin was taken up by Caco-2 cells incubated with micellar fucoxanthin composed of 1 �mol/L fucoxanthin, 2 mmol/L sodium taurocholate, 100 �mol/L monoacylglycerol, 33.3 �mol/L fatty acids and 50 �mol/L lysophosphatidylcholine. Fucoxanthinol, the deacetylated product of fucoxanthin, was also found in both medium and cells, with its level increasing significantly in a time-dependent manner. No conjugated forms of fucoxanthin and fucoxanthinol were found in either medium or cells. In the animal study, fucoxanthinol (10.4 � 5.3 nmol/L plasma, n � 4) was detected in plasma of mice 1 h after intubation of 40 nmol fucoxanthin. These results indicate that dietary fucoxanthin is incorporated as fucoxanthinol, the deacetylated form, from the digestive tract into the blood circulation system in mammals.

Item Type:	Article
Uncontrolled Keywords:	Hydrolysis; Lipase; HPLC analyses
Subjects:	600 Technology > 08 Food technology > 29 Microbiological food > 01 Algae
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K.S
Date Deposited:	21 Jun 2011 09:45
Last Modified:	28 Dec 2011 10:24
URI:	http://ir.cftri.res.in/id/eprint/10150

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