Studies on aptamer for biosensor application.

Poonam, Venkatrao Gontalwar (2011) Studies on aptamer for biosensor application. [Student Project Report] (Submitted)

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	We have successfully developed a novel colorimetric aptasensor using gold nanoparticles (GNPs) for sensitive and specific detection of vitamin B12, one of the most commonly deficient vitamin lead to pernicious anemia. Ahighly stable RNA aptamer that bind to vitamin B12 was employed by structural modification at 2’hydroxyl group of ribose to 2’ Fluoro of all pyrimidines indicated in lower case in 35 bases length aptamer (5’ GGA Acc GGu CAu AAc cAc cuc AGu GcG AGc AA 3’) (Lorsch J.R. and Szostak J.W. 1994, Biochemistry, 33:973-982). Aggregation of GNPs was specifically induced by desorption of the vitamin B12 binding RNA aptamer from the surface of GNPs as a result of the aptamer target interaction, leading to the color change from red to purple. The limit ofdetection of vitamin B12 was100 ng/ml by successful optimization on the amount of the aptamers, GNPs, salts, and stability of aptamer. This colorimetric aptasensor is advantageous over the other conventional methods, in terms of simple signal generation and detection with naked eye, which can be realized in onsite detection for other environmental and clinical samples.
Uncontrolled Keywords:	aptamers; biosensor application; colorimetric aptasensor
Subjects:	600 Technology > 02 Engineering & allied operations
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Jan 2012 10:03
Last Modified:	30 Jun 2015 06:22
URI:	http://ir.cftri.res.in/id/eprint/10556

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