Pigment identification, nutritional composition, bioactivity, and in vitro cancer cell cytotoxicity of Rivina humilis L. berries, potential source of betalains

Imtiyaj Khan, Mohammad and Sri Harsha, P. S. C. and Giridhar, P. and Ravishankar, G. A. (2012) Pigment identification, nutritional composition, bioactivity, and in vitro cancer cell cytotoxicity of Rivina humilis L. berries, potential source of betalains. LWT - Food Science and Technology, 47. pp. 315-323.

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Abstract

Ripened berries of Rivina humilis L. (pigeon berry) were investigated for pigment and nutritional composition, in vitro bioactivities, and cancer cell cytotoxicity of its extracts. Ten betalain pigments (total contente0.35 g/100 g fresh weight, and 1.7 g/100 g dry weight) with high level of betaxanthins were characterised. Carbohydrates (6.2 g), proteins (1.1 g), lipids (0.7 g), phenols (105.7 mg gallic acid equivalent), niacin (5.3 mg), and total tocopherols (0.77 mg) in 100 g fresh weight were observed. Antioxidant bioactivity of MeOH extract (maximum at 100 mg/mL) against OH� and b-carotene oxidation was studied. The extract (1000 mg/mL) effectively protected kidney lipid peroxidation compared to butylated hydroxy anisole. Betalains rich extract, and purified betacyanins and betaxanthins revealed EC50 against DPPH� (51.0, 0.29, 0.11 mg/mL), and reducing power (39.3, 2.79, and 1.34 mg/mL) which was higher than that of gallic acid and ascorbic acid. In vitro cancer cell cytotoxicity was assessed through MTT assay on HepG2 cells after exposing to betalains rich extract, betacyanins and betaxanthins for 24 h; only betaxanthins exhibited cytotoxicity (EC50 12.0 mg/mL). After 48 h of exposure, betacyanins and betaxanthins showed elevated cytotoxicity.

Item Type:	Article
Uncontrolled Keywords:	Rivina humilis L. (Phytolaccaceae), Betalains Cytotoxicity, Bioactivity, Lipid peroxidation
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 08 Food technology > 16 Nutritive value
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 May 2012 04:33
Last Modified:	11 Oct 2018 08:34
URI:	http://ir.cftri.res.in/id/eprint/10781

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