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Separation and Purification of Bromelain by Reverse Micellar Extraction Coupled Ultrafiltration and Comparative Studies with Other Methods
Umesh Hebbar, H. and Sumana, B. and Hemavathi, A. B. and Raghavarao, K. S. M. S. (2012) Separation and Purification of Bromelain by Reverse Micellar Extraction Coupled Ultrafiltration and Comparative Studies with Other Methods. Food and Bioprocess Technology, 5. pp. 1010-1018.
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Food and Bioprocess Technology, 2012, Volume 5, Number 3, Pages 1010-1018.pdf - Published Version Restricted to Registered users only Download (239kB) |
Abstract
Abstract Reverse micellar extraction (RME) is a promising liquid-liquid extraction technique for downstream processing of biomolecules from dilute solutions. An integrated approach of coupling RME with ultrafiltration is attempted to improve the overall efficiency of extraction and purification of bromelain from aqueous extract of pineapple core. The performance of RME is compared with aqueous two-phase extraction (ATPE), another potential liquid-liquid extraction technique and conventional ammonium sulphate precipitation technique. The reverse micellar system of cationic surfactant cetyltrimethylammoniumbromide/isooctane/hexanol/butanol used for RME resulted in an activity recovery of 95.8% and purification of 5.9-fold. The purification of bromelain increased to 8.9-fold after ultrafiltration. Alteration of aqueous phase pH during RME facilitated the differential partitioning of bromelain and polyphenoloxidase. Comparison of RME results with ATPE (activity recovery of 93.1% and purification of 3.2-fold) and the conventional ammonium sulphate precipitation (activity recovery of 82.1% and purification of 2.5-fold) indicated the improved performance of RME.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Activity recovery ATPE Bromelain Partition coefficient Reverse micelles Ultrafiltration |
| Subjects: | 600 Technology > 08 Food technology > 07 Food Engineering |
| Divisions: | Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 25 May 2012 10:59 |
| Last Modified: | 11 Oct 2018 09:25 |
| URI: | http://ir.cftri.res.in/id/eprint/10790 |
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