Isolation, purification, and some of the properties of hemi-cellulase from fusarium sp.

Dharmaraj, B. Wankhede and Vijayalakshmi, K. R. and Madhava Rao, R. and Raghavendra, Rao (1981) Isolation, purification, and some of the properties of hemi-cellulase from fusarium sp. Carbohydrate Research, 96 (2). pp. 249-257.

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Abstract

An extracellular hemicellulase from a soil fungus (Fusarium sp.) grown on a medium containing groundnut hemicellulose B was purified 76-fold by ammonium sulphate fractionation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-100. It was found to be homogeneous by disc-gel electrophoresis at pH 8. It showed optimal activity at pH 5.6 and 37°. It was observed that groundnut and sesame hemicellulose B were degraded considerably (~80 and 58%, respectively) by the purified hemicellulase, whereas glucomannan and xylan from groundnut were comparatively poorly hydrolysed (~30–40%).

Item Type:	Article
Uncontrolled Keywords:	soil fungus, hemicellulose B
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Feb 2018 10:52
Last Modified:	22 Feb 2018 10:52
URI:	http://ir.cftri.res.in/id/eprint/10810

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