Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

Rajeev, Ranjan and Rastogi, N. K. and Thakur, M. S. (2012) Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide. Journal of Hazardous Materials, 225. pp. 114-123. ISSN 0304-3894

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Abstract

The present communication deals with construction of immobilized robust biophotonic bead using P. leiognathi, a marine luminescent bacterium for their possible application in monitoring of environmental toxicants. Immobilization efficiency of agar, carrageenan and sodium alginate was evaluated separately in terms of luminescence response and was recorded as 30.3, 77.4 or 99.5%, respectively. Under optimized storage conditions, the luminescent response of P. leiognathi in the immobilized state was studied over a period of 30 days. These biophotonic beads were further used as a rapid and reliable optical biosensing tool for the detection of heavy metals [Hg(II), As(V) or Cd(II)] and pesticide [2,4-dichlorophenoxyacetic acid (2,4-D)] in water systems. The concentration range for the detection of Hg(II), As(V), Cd(II) and 2,4-D was 2–32 ppm, 4–128 ppm, 16–512 ppm and 100–600 ppm, respectively, while corresponding sensitivity threshold was 2.0 ppm, 4.0 ppm, 16.0 ppm and 100 ppm. A comparison of inhibition constant (Kd) (or EC20) values indicated that the sensitivity thresholds rank as Hg(II) > As(V) > Cd(II) > 2,4-D. Moreover, the time taken for the detection of heavy metals and pesticide was less than 30 min. Using the bioluminescence inhibition method, the concentration of heavy metals and pesticide could be predicted.

Item Type:	Article
Uncontrolled Keywords:	P. leiognathi, Immobilization, Biophotonic bead Environmental toxicants, Bioluminescence inhibition
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 06 Marine Microbiology
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2012 07:03
Last Modified:	29 Oct 2018 09:26
URI:	http://ir.cftri.res.in/id/eprint/10830

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