Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System.

Lakshmi, M. C. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2012) Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System. Food and Bioprocess Technology, 5. pp. 193-199.

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Abstract

The purification of lipoxygenase in soybeans (richest known source) was attempted by precipitation, using polyethylene glycol, followed by aqueous two-phase extraction (ATPE). The preliminary purification by precipitation was standardized which resulted in two-fold purification. The influence of process parameters such as phase forming salt, molecular weight of phase forming polymer, tie line length, and phase volume ratio on the partitioning behavior of lipoxygenase during ATPE was investigated. The aqueous two-phase system of polyethylene glycol 6000 and ammonium sulfate, with inherent pH of 5.2, was found to be most suitable. The tie line length of 41.25% and volume ratio of 1.7 have resulted in 125% activity recovery with the overall purification factor of 4.38 compared to crude enzyme extract.

Item Type:	Article
Uncontrolled Keywords:	Lipoxygenase . Aqueous two-phase extraction . Precipitation . Purification
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean 600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	11 Mar 2013 05:54
Last Modified:	11 Mar 2013 05:54
URI:	http://ir.cftri.res.in/id/eprint/11141

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