Integrated Approach for the Extraction and Purification of IgY from Chicken Egg Yolk.

Priyanka, B. S. and Abhijith, K. S. and Rastogi, N. K. and Raghavarao, K. S. M. S. (2014) Integrated Approach for the Extraction and Purification of IgY from Chicken Egg Yolk. Separation Science and Technology, 49. pp. 562-568.

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Abstract

The production of Immunoglobulin Y (IgY) from an immunized chicken egg yolk serves numerous applications in various fields of immunology. The present work, for the first time, demonstrates the feasibility of aqueous two-phase extraction and three phase partitioning for the separation of IgY from the chicken egg yolk. The protocol deals with partitioning of IgY using polyethylene glycol 1500/potassium phosphate aqueous twophase system and its comparison with three phase partitioning, both followed by the precipitation. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was carried out to confirm the presence of IgY at the different steps of purification process. The IgY was preferentially partitioned to the PEG-rich top phase, especially at lower phase-volume ratio. The effect of process parameters such as tie line length and volume ratio was studied. The tie line length of 5.55%and volume ratio of 0.49 gave the best results. The yield of IgY obtained from aqueous two-phase system was 9.0mg/mL of egg yolk while that of three phase partitioning it was 6.0mg/mL.

Item Type:	Article
Uncontrolled Keywords:	egg yolk; precipitation; three phase partitioning; twophase system
Subjects:	600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Meat 600 Technology > 08 Food technology > 07 Food Engineering
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Apr 2014 09:09
Last Modified:	25 Apr 2014 09:09
URI:	http://ir.cftri.res.in/id/eprint/11457

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