Immunoassay for Lead Ions: Analysis of Spiked Food Samples.

Mandappa, I. M. and Ranjini, A. and Haware, D. J. and Manonmani, H. K. (2014) Immunoassay for Lead Ions: Analysis of Spiked Food Samples. Journal of Immunoassay and Immunochemistry, 35. pp. 1-11.

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Abstract

A rapid, simple, and reliable competitive immunoassay was developed for measurement of lead ions in spiked food samples. Avian antibodies were produced against Pb(II). Since lead ions are too small to elicit an immune response, the metal was coupled to protein carrier Bovine Serum Albumin (BSA) using a bifunctional chelator 1-(4-isothiocyanobenzyl) ethylenediamine N,N,N0,N0- tetraacetic acid (ITCBE). Poultry birds (layers) were immunized with this Pb(II)–ITCBE–BSA immunoconjugate and avian antibodies (IgY) were isolated from egg yolk. This avian antibody (IgY) produced recognized Pb(II)-ITCBE complexes as capture reagent. The assay depended on a competitive binding reaction between the antibody and Pb(II). Antibody reaction was optimized for different concentrations of antigen and antibody dilutions. Cross reactivity with other metals were below 1% in competitive ELISA. The detection range and the detection limit were 0.02–1000 mg � kg�1 and 0.2 mg � kg�1, respectively. Spike recovery studies in different food samples showed that the avian antibodies could recognize Pb(II) in food samples without much matrix effect.

Item Type:	Article
Uncontrolled Keywords:	avian antibodies (IgY), cross reactivity, detection of Pb(II), ELISA, food samples, specificity
Subjects:	600 Technology > 08 Food technology > 13 Quality control
Divisions:	Fermentation Technology and Bioengineering Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Apr 2014 09:36
Last Modified:	25 Apr 2014 09:36
URI:	http://ir.cftri.res.in/id/eprint/11463

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