Evaluating the effect and effectiveness of different constructs with a conserved sequence for silencing of Coffea canephora N-methyltransferases.

Shibin, Mohanan and Satyanarayana, K. V. and Sridevi, V. and Kalpashree, Gowda (2014) Evaluating the effect and effectiveness of different constructs with a conserved sequence for silencing of Coffea canephora N-methyltransferases. Journal of Plant Biochemistry and Biotechnology, 23 (4). pp. 399-409.

PDF Journal of Plant Biochemistry and Biotechnology October 2014, Volume 23, Issue 4, pp 399-409.pdf - Published Version Restricted to Registered users only Download (749kB)

Abstract

Caffeine biosynthesis involves sequential methylation of the purine ring by the transfer of methyl group from the methyl donor S-adenosyl methionine. Genes that code for the individual methyl transferase have a high degree of sequence homology. To achieve decaffeination by genetic engineering of coffee, the effect and efficiency of different post-transcriptional gene silencing (PTGS) constructs were evaluated. Sense, antisense and invert repeat constructs were developed using a 339 bp fragment from the conserved region of the coffee Nmethyltransferase genes involved in caffeine biosynthesis.

Item Type:	Article
Uncontrolled Keywords:	Coffee Coffea canephora Genetic transformation Caffeine biosynthesis Decaffeination Gene silencing
Subjects:	600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering 600 Technology > 07 Beverage Technology > 04 Coffee
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Oct 2014 06:56
Last Modified:	16 Oct 2014 06:56
URI:	http://ir.cftri.res.in/id/eprint/11649

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