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Assessment of Acrylamide Degradation Potential of Pseudomonas aeruginosa BAC-6 Isolated from Industrial Effluent.

Vijayashree, Chandrashekar and Chandrika, Chandrashekar and Rajath, Shivakumar and Sourav, Bhattacharya and Arijit, Das and Bhaskar, Gouda (2014) Assessment of Acrylamide Degradation Potential of Pseudomonas aeruginosa BAC-6 Isolated from Industrial Effluent. Applied Biochemistry and Biotechnology, 173 (5). pp. 1135-1144.

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Acrylamide finds diverse industrial applications but is considered an environmental threat because of its neurotoxic, carcinogenic, and teratogenic effects. Certain bacteria enzymatically degrade acrylamide to acrylic acid and ammonia. The present investigationwas carried out to isolate and identify an acrylamide-degrading bacterium from industrial effluent. Bacterial growth and extent of acrylamide degradation in the presence of different acrylamide concentrations, nutrients, varied range of pH, and temperature were analyzed.Among the eight acrylamide-degrading isolates, isolate BAC-6 demonstrated the highest degradation, and based upon the partial 16S rDNA sequencing, it was identified as Pseudomonas aeruginosa. P. aeruginosa BAC-6 grew over a wide range of acrylamide concentrations, but the highest degradationwas recorded at 500 mg/L concentrationwith concomitant cell growth. Among the carbon supplements, mannitol supported the highest growth and degradation. Maximum degradation was reported at neutral pH. A mesophilic temperature range (25–40 °C) facilitated conducive bacterial growth followed by degradation. The highest degradation and bacterial growth were observed at 30 and 35 °C, respectively. Thus, it could be inferred from the present investigation that cultural conditions strongly affected the degradation potential of P. aeruginosa BAC-6 and advocated the utilization of the isolate in bioremediation of sites polluted with acrylamide.

Item Type: Article
Uncontrolled Keywords: Acrylamide . Pseudomonas aeruginosa . Degradation . High-performance liquid chromatography
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
600 Technology > 01 Medical sciences > 17 Toxicology
Divisions: Lipid Science and Traditional Foods
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jun 2015 09:54
Last Modified: 12 Jun 2015 09:54
URI: http://ir.cftri.res.in/id/eprint/11779

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