Molecular identification and characterization of Fusarium spp. associated with sorghum seeds.

Divakara Shetty, T. and Parthasarathy, Santosh and Mohammed, Aiyaz and Mudili, Venkata Ramana and Hariprasad, P. (2014) Molecular identification and characterization of Fusarium spp. associated with sorghum seeds. Journal of the Science of Food and Agriculture, 94. pp. 1132-1139.

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Abstract

BACKGROUND: Fusarium spp. are not only pathogenic to plants but are also known as toxin producers that negatively affect animal and human health. The identification of Fusarium spp. remains one of the most critical issues in fungal taxonomy. In this study, different strains of Fusarium spp. were isolated from sorghum seed samples and identified at themolecular level by tef-1α gene amplification. A multiplex polymerase chain reaction (mPCR) assay was developed to differentiate toxigenic and non-toxigenic Fusarium spp. by designing a primer for the Fum21 gene along with the Fum1 and Fum8 genes. A competitive direct enzyme-linked immunosorbent assay (CD-ELISA) was employed to assess the fumonisin-producing ability of Fusarium spp. Phylogenetic analyses were performed using partial sequences of tef-1α and inter-simple sequence repeat (ISSR)markers of different Fusarium spp. RESULTS: All 27 isolates of Fusarium spp. were positive for the tef-1α gene and revealed the presence of F. verticillioides, F. thapsina and F. cf. incarnatum-equiseti complex. The standardized mPCR assay distinguished toxigenic and non-toxigenic F. verticillioides. Further, mPCR fumonisin-positive F. verticillioides isolates were also positive by CD-ELISA. The tef-1α gene sequence was found to be useful in revealing intraspecific polymorphism to some extent. ISSR markers revealed a high level of polymorphism among different isolates of Fusarium spp., and the dendrogram of ISSR analyses grouped the 27 isolates into twomajor clusters. CONCLUSION: The presentmethodprovided rapid andreliable detection of fumonisin-producing Fusarium spp. ThemPCRassay couldbe analternative strategy to current conventional mycotoxin analytical techniques anda reliable tool forhigh-throughput monitoring of major mycotoxin-producing fungi during the processing steps of food and feed commodities.

Item Type:	Article
Uncontrolled Keywords:	sorghum; Fusarium spp; tef-1α gene; ISSR markers
Subjects:	600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum 600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Jun 2015 07:42
Last Modified:	29 Jun 2015 06:39
URI:	http://ir.cftri.res.in/id/eprint/11804

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