Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus

Mandappa, I. M. and Prasanna, Joglekar and Manonmani, H. K. (2015) Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus. Journal of Food Science and Technology, 52 (7). pp. 4642-4646.

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Abstract

A multiplex real-time isothermal amplification assay was developed using molecular beacons for the detection of Bacillus cereus and Staphylococcus aureus by targeting four important virulence genes. A correlation between targeting highly accessible DNA sequences and isothermal amplification based molecular beacon efficiency and sensitivity was demonstrated using phi(Φ)29 DNA polymerase at a constant isothermal temperature of 30 °C. It was very selective and consistently detected down to 101 copies of DNA. The specificity and sensitivity of this assay, when tested with pure culture were high, surpassing those of currently used PCR assays for the detection of these organisms. The molecular beacon based real-time isothermal amplification (MBRTIA) assay could be carried out entirely in 96 well plates or well strips, enabling a rapid and high-throughput detection of food borne pathogens.

Item Type:	Article
Uncontrolled Keywords:	Detection . Food borne pathogens . B. cereus . S. aureus . Molecular beacon
Subjects:	600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	17 Feb 2016 06:07
Last Modified:	17 Feb 2016 06:07
URI:	http://ir.cftri.res.in/id/eprint/12095

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