Diet-induced hypercholesterolemia imparts structure function changes to erythrocyte chondroitin sulphate/dermatan sulphate

Kiran, G. and Srikanth, C. B. and Salimath, P. V. and Nandini, C. D. (2015) Diet-induced hypercholesterolemia imparts structure function changes to erythrocyte chondroitin sulphate/dermatan sulphate. Journal of Biochemistry, 3. pp. 1-8.

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Abstract

Hypercholesterolemia is one of the factors contributing to cardiovascular problems. Erythrocytes are known to contribute its cholesterol to atherosclerotic plaque. Our earlier study showed that erythrocytes overexpress chondroitin sulphate/dermatan sulphate (CS/DS), a linear co-polymer, during diabetes which resulted in increased cytoadherence to extracellular matrix (ECM) components. This study was carried out to determine whether diet-induced hypercholesterolemia had any effect on erythrocyte CS/DS and impacted cytoadherence to ECM components. Unlike in diabetes, diet-induced hypercholesterolemia did not show quantitative changes in erythrocyte CS/DS but showed difference in proportion of un-sulphated and 4-Osulphated disaccharides. Erythrocytes from hypercholesterolemic rats showed increased adhesion to ECM components which was abrogated to various extents when subjected to chondroitinase ABC digestion. However, isolated CS/DS chains showed a different pattern of binding to ECM components indicating that orientation of CS/DS chains could be playing a role in binding.

Item Type:	Article
Uncontrolled Keywords:	chondroitin sulphate dermatan sulphate erythrocytes extracellular matrix hypercholesterolemia.
Subjects:	600 Technology > 08 Food technology > 11 Food Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	18 May 2016 07:00
Last Modified:	18 May 2016 07:00
URI:	http://ir.cftri.res.in/id/eprint/12152

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