In vitro evaluation of the cholesterol-reducing ability of a potential probiotic Bacillus spp

Shobharani, P. and Prakash, M. Halami (2016) In vitro evaluation of the cholesterol-reducing ability of a potential probiotic Bacillus spp. Annals of Microbiology, 66. pp. 643-651.

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Abstract

The cholesterol removal ability of three potential probiotic Bacillus spp., namely, B. flexusMCC 2458, B. flexus MCC 2427 and B. licheniformis MCC 2514, has been studied. All the three isolates were deprived of bile salt hydrolase enzyme, but they were still able to remove cholesterol by other mechanisms which were strain-dependent. B. flexus MCC 2427 used cholesterol oxidase (2.5 U/mL) for cholesterol removal, whereas B. flexus MCC 2458 underwent coprecipitation in the presence of bile. B. licheniformis MCC 2514 was involved in cholesterol assimilation, which was also confirmed through an increase in saturated and unsaturated fatty acid contents in the cellular lipid profile. An interesting observation was that the cell-free supernatants of all three isolates exhibited cholesterol-reducing ability and that all were stable at both acidic and alkaline pH. Although the cultures were observed to differ in behavior, their hypocholesterolemic effect satisfies a criterion for their potential application in the food and pharmaceutical industries.

Item Type:	Article
Uncontrolled Keywords:	Bacillus spp. . Co-precipitation . Probiotic . Cholesterol oxidase . Fatty acid profile
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 May 2016 10:42
Last Modified:	30 May 2016 10:42
URI:	http://ir.cftri.res.in/id/eprint/12227

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