Maternal and neonatal dietary intake of balanced n‑6/n‑3 fatty acids modulates experimental colitis in young adult rats

Vijay Kumar Reddy, K. and Akhilender Naidu, K. (2016) Maternal and neonatal dietary intake of balanced n‑6/n‑3 fatty acids modulates experimental colitis in young adult rats. European Journal of Nutrition, 55. pp. 1875-1890.

PDF Eur J Nutr (2016) 551875–1890.pdf - Published Version Restricted to Registered users only Download (3MB)

Abstract

Background The imbalance of n-6 and n-3 polyunsaturated fatty acids in the maternal diet impairs intestinal barrier development and sensitizes the colon response to inflammatory insults in the young rats. With a view to overcoming this issue, we designed this study to investigate the effect of maternal and neonatal intake of different proportions of n-6/n-3 fatty acids on colon inflammation in the young adult rats. Methods Female Wistar rats were assigned into four groups, and each group fed one of four semisynthetic diets, namely n-6, low n-3, n-6/n-3 and n-3 fatty acids for 8 weeks prior to mating, during gestation and lactation periods. At weaning, the pups were separated from the dams and fed diet similar to the mothers. Colitis was induced on postnatal day 35, by administering 2 % dextran sulfate sodium in drinking water for 10 days. Colitis was assessed based on the clinical and inflammatory markers in the colon. Fatty acid analysis was done in liver, RBC, colon and spleen. Results A balanced n-6/n-3 PUFA diet significantly improved the body weight loss, rectal bleeding and mortality in rats. This was associated with lower myeloperoxidase activity, nitric oxide, prostaglandin E2, TNF-α and IL-6, IL-8, COX-2 and iNOS levels in the colon tissues. Fatty acid analysis has shown that the arachidonic acid/ docosahexaenoic acid ratio was significantly lower in liver, RBC, colon and spleen in n-6/n-3 and n-3 diet groups. Conclusion We demonstrate that balanced n-6/n-3 PUFA supplementation in maternal and neonatal diet alters systemic AA/DHA ratio and attenuates colon inflammation in the young adult rats.

Item Type:	Article
Uncontrolled Keywords:	Ulcerative colitis · Maternal and neonatal diet · Garden cress oil · Inflammatory mediators
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Aug 2016 12:20
Last Modified:	27 Aug 2018 04:25
URI:	http://ir.cftri.res.in/id/eprint/12262

Actions (login required)

View Item