Approaches for Biological Improvement of Eco-friendly Crops

Priyadarshi, S. and Anubha, Shukla and Borse, B. B. (2013) Approaches for Biological Improvement of Eco-friendly Crops. Journal of Biological and Scientific Opinion, 1 (3). pp. 243-249.

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Abstract

Marker gene is a DNA sequence whose phenotype can be easily recognized and identified. It can be used to flag another gene. A selective marker encourages the growth in the presence of a selective agent, which kills or prevents the growth of a cell. Antibiotics resistance gene is used to tag target gene or gene carrying a trait of interest. Antibiotics are used in crops during transfer of gene of interest in crops improvement programm es. Antibiotics and antibiotics resistance gene has been used for a long time in plant biotechnology. Not even one Gene transfer method has so much efficiency so that screening allows the selection of transformed cells having marker gene together with targ et gene which inactivates the antibiotic and protect the cells from antibiotic while non transformed cells do not have marker gene so they are killed in presence of antibiotics during screening.

Item Type:	Article
Uncontrolled Keywords:	Cre/lox crossing, FLP/FRT, R/RS System, GUS gene, H omologous recombination system
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology 600 Technology > 03 Agriculture
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Sep 2016 10:48
Last Modified:	23 Sep 2016 10:48
URI:	http://ir.cftri.res.in/id/eprint/12322

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