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Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death
Darshan, N. and Manonmani, H. K. (2016) Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death. AMB Express, 6. pp. 50-62.
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Abstract
The antimicrobial activity of prodigiosin from Serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The mode of action of prodigiosin was studied. Prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of Programmed cell death (PCD). PCD involves DNA fragmentation, generation of ROS, and expression of a protein with caspase-like substrate specificity in bacterial cells. Prodigiosin was observed to be internalized into bacterial cells and was localized predominantly in the membrane and the nuclear fraction, thus, facilitating intracellular trafficking and then binding of prodigiosin to the bacterial DNA. Corresponding to an increasing concentration of prodigiosin, the level of certain proteases were observed to increase in bacteria studied, thus initiating the onset of PCD. Prodigiosin at a sub-inhibitory concentration inhibits motility of pathogens. Our observations indicated that prodigiosin could be a promising antibacterial agent and could be used in the prevention of bacterial infections.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Prodigiosin, Serratia nematodiphila, Bactericidal activity, Programmed cell death, Motility |
| Subjects: | 600 Technology > 08 Food technology > 10 Food Microorganisms |
| Divisions: | Food Protectants and Infestation Control |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 08 Nov 2016 07:40 |
| Last Modified: | 08 Nov 2016 07:40 |
| URI: | http://ir.cftri.res.in/id/eprint/12416 |
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