Isolation of anti-listerial bacteriocin producing Lactococcus lactis CFR-B3 from Beans (Phaseolus vulgaris).

Kiran Kumar, P. and Badarinath, V. and Prakash, M. Halami (2008) Isolation of anti-listerial bacteriocin producing Lactococcus lactis CFR-B3 from Beans (Phaseolus vulgaris). The Internet Journal of Microbiology, 6 (1). pp. 1-6.

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Abstract

Contamination of food samples by Listeria monocytogenes is a major cause of food-borne infections. Controlling the growth of Listeria sp. by application of bacteriocin or bacteriocin producing-organism in foods is one of the challenges in the development of effective bio-preservation process. The objective of this study was to isolate and to characterize bacteriocinogenic strain Lactococcus lactis CFR-B3 from Indian beans. Bacterial strain of Pediococcus acidilactici NRRL B1153 was used as indicator to screen bacteriocin production. The culture was found to produce class IIa heat-stable anti-listerial bacteriocin of MW ~ 5 kDa. The use of food-grade organism as an indicator for screening bacteriocinogenic strains of lactic acid bacteria circumvent the need for using pathogenic cultures such as listeria spp. The native isolate Lc. lactis CFR-B3 finds its application in the preservation of vegetables due to its prevalence in the same ecological niche.

Item Type:	Article
Uncontrolled Keywords:	Lactococcus lactis, Listeria sp., bacteriocin, Indian beans, Green beans
Subjects:	600 Technology > 08 Food technology > 10 Food Microorganisms 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Dec 2017 09:15
Last Modified:	19 Dec 2017 09:15
URI:	http://ir.cftri.res.in/id/eprint/13211

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