Neuromodulatory Potential of Aqueous Extracts of Cumin, Cinnamon: Evidence from Rotenone Model in Drosophila: Implications to Parkinson’s Disease.

Sriranjini, Venkata Rao and Greeshma, M. and Dr., Muralidhara and Rajini, P. S. (2016) Neuromodulatory Potential of Aqueous Extracts of Cumin, Cinnamon: Evidence from Rotenone Model in Drosophila: Implications to Parkinson’s Disease. International Journal of Neurology Research, 2 (3-4). pp. 297-307.

PDF Int. J. Neurology Res. 2016 December; 2(3-4) 297-307.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

AIM: Spice extracts and their bioactive molecules have been well recognized for their innumerable beneficial effects against various chronic diseases. However, experimental data regarding their potential to abrogate oxidative stress and neurotoxicity in animal models of Parkinson’s disease (PD) are rather limited. In the current study, we aimed to assess the neuromodulatory potential of aqueous extracts of spices viz., cumin and cinnamon and their bioactives (Cuminaldehyde (CU) and Cinnamaldehyde (CN) using a rotenone (ROT) model of neurotoxicity in Drosophila. MATERIALS AND METHODS: Adult male flies (Oregon K) were fed medium enriched (0.1-0.2%) with aqueous extract of Cumin or Cinnamon and their bio-actives (CU/ CN) with without ROT (500 μM) for 7 days. The propensity of extracts or bio-actives to protect flies against ROT-induced lethality, locomotor phenotype, oxidative stress and neurotoxicity was determined. While both the extracts significantly protected the flies against ROT-induced mortality, the survivors exhibited improved locomotor phenotype. Further both CU and CN-enrichment markedly reduced the ROT -induced lethality, diminished locomotor deficits and significantly abrogated the degree of oxidative impairments. Both bio-actives also augmented the antioxidant enzyme activities and restored ROT-induced mitochondrial dysfunctions. Interestingly, ROT -induced elevation of the activity of acetylcholinesterase and depletion of dopamine levels were also restored. Further, flies provided prophylactic treatment with bio-actives exhibited significant resistance to an acute exposure to Paraquat (PQ). In a parallel study, both bio-actives were found to significantly delay the onset of locomotor deficits among ROT-stressed flies besides extending their survival. CONCLUSION: We hypothesise that the efficacy aqueous extract and their bioactives to attenuate ROT-mediated neurotoxicity may be largely related to the combined antioxidant activity of bio-actives resulting in improved locomotor performance, abrogation of oxidative stress and mitochondrial dysfunction. Based on these results, we propose that cumin and cinnamon extracts may be exploited as therapeutics against PD and other neurodegenerative diseases.

Item Type:	Article
Uncontrolled Keywords:	Cuminaldehyde; Cinnamaldehyde; Drosophila; Rotenone; Oxidative stress; Neurotoxicity; Parkinsonism
Subjects:	600 Technology > 08 Food technology > 30 Spices/Condiments 600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Dept. of Biochemistry Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 Jan 2018 06:16
Last Modified:	09 Jan 2018 06:16
URI:	http://ir.cftri.res.in/id/eprint/13250

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