Hypolipidemic mechanism of oryzanol components- ferulic acid and phytosterols.

Bhaskaragoud, G. and Rajath, Shivakumar and Mahendra, V. P. and Sunil Kumar, G. and Gopala Krishna, A. G. and Suresh Kumar, G. (2016) Hypolipidemic mechanism of oryzanol components- ferulic acid and phytosterols. Biochemical and Biophysical Research Communications, 476 (2). pp. 82-89.

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Abstract

The effect of oryzanol (well known hypolipidemic component in rice bran oil) and its chemical constituents- ferulic acid (FA) and phytosterols on hypolipidemia were investigated. METHODS AND RESULTS: Docking (in silico) studies showed that FA had a better binding ability with lipase while sterols bound well with HMG-CoA reductase. Further in vivo studies of feeding high fat (30%) to rats increased body weights, serum TC, TG, non-HDL-C and reduced HDL-C were observed, compared to normal diet fed group (ND). ORZ treated groups alleviated the lipid profile. Furthermore, increased organ weights, higher intestinal lipase activity, and liver lipid peroxidation was observed in the high-fat group (HF). These effects were ameliorated in oryzanol concentrate fed groups (ORZ). Higher fecal fat was found in ORZ groups, analysis of fecal matter by mass spectroscopy revealed the presence of FA. In vitro, a bile acid binding study supported the strong affinity of sterol towards bile acids. In conclusion, oryzanol in the intestine is cleaved into FA and sterol by intestinal lipase enzymes both lipase and HMG-CoA reductase activities were inhibited, respectively. These hydrolysates eliminated the bile acids, thus lowering lipid profiles.

Item Type:	Article
Uncontrolled Keywords:	Ferulic acid; HMG-CoA reductase; Lipase; Molecular docking; Oryzanol; Sterols
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 22 Lipid Chemistry
Divisions:	Dept. of Biochemistry Lipid Science and Traditional Foods
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jan 2018 05:40
Last Modified:	22 Jan 2018 05:40
URI:	http://ir.cftri.res.in/id/eprint/13357

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