Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HSSPME-GC) method for the analysis of zingiber zerumbet L.

Bhavya, M. L. and Ravi, R. and Madhava Naidu, M. (2019) Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HSSPME-GC) method for the analysis of zingiber zerumbet L. Natural Product Research.

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Abstract

Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of Zingiber zerumbet L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7lm), polyacrylate (PA, 85lm)], different temperature and time on the HS-SPME-GC was investigated by using response surface methodology coupled with full factorial experimental design. The area percentage of the major sesquiterpenes (Zerumbone and a-humulene) were 56.3±4.7% and 47.5±27.2% with PA fiber, respectively at optimum condition of 70�C and 30min. ValidationofthedevelopedHS-SPME-GCmethodwithlimitsofdetection and quantification for zerumbone was 0.09 and 0.28lg/g, respectively, demonstrating the suitable sensitivity of HS-SPME-GC methodforthequantificationofsesquiterpenes.Therefore,thesimplicity of HS-SPME-GC makes it a convenient tool for qualitative and quantitative comparison of different ginger species targeting at the markersesquiterpenemolecule,zerumbone.

Item Type:	Article
Uncontrolled Keywords:	Headspace solid-phase microextraction; Zingiber zerumbet; Zerumbone; Gas chromatography; Optimization
Subjects:	600 Technology > 08 Food technology > 01 Analysis
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Feb 2020 11:08
Last Modified:	27 Jul 2020 08:56
URI:	http://ir.cftri.res.in/id/eprint/14224

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