Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.

Deepesh, Panwar and Mukesh, Kapoor (2020) Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiology, 86. pp. 1-8.

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Abstract

Plantderivedgalactomannooligosaccharides(GMOS)areanemergingclassofprebiotics,butnoinformationis available on theirutilization in lactobacilli atthe molecular level. Thecurrent studyaimed atidentifying the geneticlociinvolvedinthetransportandcatabolismoflocustbeangumderivedGMOSinLactobacillusplantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degreeofpolymerization;DP≤3).GlobaltranscriptomemicroarrayprofilingofL.plantarumWCFS1revealed differential expression when GMOS or control sugars (glucose, galactose, and mannose) were used as a sole carbohydratesource.Twogeneticlociinvolvedincellobiose(~3.2kb)andoligo-sucrose(~7.3kb)utilizationin L. plantarumWCFS1werehighlyup-regulatedupto8.3 andupto6.7-fold,respectivelyby GMOSutilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptomeandqRT-PCRstudiesofL.plantarumWCFS1suggestedthatun-substitutedmannobiose(DP2)mightbe metabolized by proteins encoded by the cellobiose operon while, substituted DP2 (galactomannose) and DP3 (galactomannobiose)weremostlikelytransportedandcatabolizedbytheoligo-sucroseutilizationlociencoded proteins.

Item Type:	Article
Uncontrolled Keywords:	Lactobacillus plantarum WCFS1 Galactomannooligosaccharides Transcriptome Operon PTS transporter Glycoside hydrolase
Subjects:	600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Mar 2020 07:15
Last Modified:	10 Mar 2020 07:15
URI:	http://ir.cftri.res.in/id/eprint/14229

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