Zn2+ stapling of N and C-terminal maintains stability and substrate affinity in GH26 endo-mannanase.

Gaurav Singh, Kaira and Usharani, D. and Mukesh, Kapoor (2020) Zn2+ stapling of N and C-terminal maintains stability and substrate affinity in GH26 endo-mannanase. Enzyme and Microbial Technology, 135. ISSN 0141-0229

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Abstract

Metal binding sites are present in one-third of proteins and are crucial for biological functions and structural maintenance. GH26 endo-mannanase (ManB-1601) from Bacillus sp. harbors a Zn2+ binding site which connects N (H1, H23) and C (E336)-terminal residues. Present study reveals how native circularization of ManB-1601 through Zn2+ coordination regulates the structure-function. We generated individual Zn2+ coordinating mutants and characterized them using biochemical and biophysical approaches. Contribution of individual Zn2+ coordination towards maintaining ManB-1601 stability and rigidity was in the following order H23>H1 > E336. Elimination of E336 and H23-Zn2+ coordination affected substrate hydrolysis to a greater degree than H1-Zn2+ coordination. Metal quantification of mutant proteins indicated that H23A did not contain Zn2+. Molecular dynamic simulation studies revealed disruption of H23-Zn2+ coordination leads to increased flexibility of N and C-terminal, active site loops and consequent drifting of substrate away from the active site region. Finally, mechanistic understanding on the functioning of Zn2+ site in ManB-1601 is developed wherein 1) H23 by anchoring Zn2+ ion majorly regulates the structure-function properties, 2) H1 provides thermostability, 3) E336 contributes towards maintaining substrate hydrolysis.

Item Type:	Article
Uncontrolled Keywords:	GH26 endo-mannanase Zn2+ binding site Protein stability Substrate affinity Molecular dynamics
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions:	Food Safety Analytical Quality Control Lab Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Jun 2020 06:25
Last Modified:	15 Jun 2020 06:25
URI:	http://ir.cftri.res.in/id/eprint/14388

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