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Monocrotophos, an organophosphorus insecticide, induces cortical and trabecular bone loss in Swiss albino mice.
Shaheen Jafri, Ali (2020) Monocrotophos, an organophosphorus insecticide, induces cortical and trabecular bone loss in Swiss albino mice. Chemico-Biological Interactions. ISSN 0009-2797
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Abstract
Till now monocrotophos (MCP) has been addressed as a neurotoxic stressor. Limited studies investigate its aftermath on bone pathologies. Given the fact that MCP is a propensely used insecticide in developing countries, this study investigates its potential to mirror osteoporotic features and bone loss incurred in a rodent model. Briefly, Swiss albino mice were intubated daily with varying doses of MCP for 8 weeks. Musculoskeletal changes were analyzed through micro-computed tomography and histology. A series of in vitro and ex vivo cell culture experiments were performed on MC3T3E-1 and primary osteoclast cultures. Results highlight that oral gavaging with MCP causes bone loss from the cortico-trabecular interface by decreasing the osteoblast and increasing the osteoclast number. Results from in vitro studies establish that MCP treatment increases the TRAP-positive multinucleated cell number during osteoclast differentiation. Ex-vivo experiments with MCP-treated animal sera further substantiate the in vivo claims with significant decreases seen in cell viability, proliferation, mineralization and differentiation studies. In conclusion MCP induces osteoclastogenesis (bone loss) on direct stimulation and alters the circulating factors in MCP-treated serum. Holistically, this work would be of potential significance to patients suffering from pesticide induced osteoporosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Monocrotophos, TRAP staining, histomorphometry, micro-CT, osteoporosis |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 30 Sep 2020 10:35 |
| Last Modified: | 30 Sep 2020 10:35 |
| URI: | http://ir.cftri.res.in/id/eprint/14470 |
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