Aqueous two-phase extraction for downstream processing of amyloglucosidase

Tanuja, S. and Srinivas, N. D. and Raghavarao, K. S. M. S. and Gowthaman, M. K. (1997) Aqueous two-phase extraction for downstream processing of amyloglucosidase. Process Biochemistry, 32 (8). pp. 635-641.

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Abstract

A polymer/salt aqueous two-phase system has been successfully employed for separation and purification of amyloglucosidase. The effects of system pH, molecular weight of polymer and composition of the two-phase system on amyloglucosidase partition behaviour in polyethylene glycol (PEG 4000, 6000)/disodium hydrogen phosphate were investigated. Experimental data are explained based on Kim's theoretical model for the prediction of biomolecule partitioning in a PEG/salt system.

Item Type:	Article
Uncontrolled Keywords:	two aqueous phase amyloglucosidase downstream processing starch hydrolysis
Subjects:	600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Sep 2007 11:26
Last Modified:	30 Jun 2015 06:31
URI:	http://ir.cftri.res.in/id/eprint/1448

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