Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis.

Niharika, Shanker and Neha, S. Berde and Govindaraju, K. and Sukumar, Debnath (2018) Efficacy of Various Methods of Protein Extraction from Purslane Suitable for Proteome Analysis. International Journal of Research and Analytical Reviews, 5 (4). pp. 451-456.

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Abstract

The purslane leaf is characterized by high content of protein, vitamins and minerals. However, its use is limited due to the high fiber content and anti-nutritional substances. In this perspective, the objective of this research was to optimize the protein extraction processes, aiming the use of purslane leaves as an alternative protein. The important characteristics of the protocol include, 1) coagulation of proteins with mild acid precipitation, 2) extraction of protein by three-phase partitioning, 3) extraction of protein by TCA/Acetone method. The protein concentrations among three methods were 802±0.2 µg/g in mild acid precipitation, 625±0.3 µg/g in TCA Acetone and 81.6±0.2 µg/g in TPP, respectively. The solubilization of proteins (method 1) showed a higher extraction yield. Based on the literature survey, the comparison of protocol for protein extraction from purslane leaf was demonstrated first time in this work and showed reproducible results.

Item Type:	Article
Uncontrolled Keywords:	Leaf protein concentrate, Essential Amino Acids, Proteome, TPP, Purslane
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 23 Vegetables
Divisions:	Food Engineering Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Oct 2020 06:37
Last Modified:	08 Oct 2020 06:37
URI:	http://ir.cftri.res.in/id/eprint/14527

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