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In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots.
Sravan Kumar, Sandopu and Giridhar, P. (2021) In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots. Plant Cell, Tissue and Organ Culture, 144 (2). pp. 477-483.
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Abstract
In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA)+N6 – Benzylaminopurine (BAP) (0.25+2.0 mg/L) and BAP+Kinetin (Kin) (2.0+0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP+Kin and IAA+BAP respectively. When compared with silver nitrate (AgNO3) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48±2.42), elongation (15.64±2.42 cm) and root length (14.52±2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the feld conditions wherein 90% success rate was noted.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Micropropagation · Adventitious rooting · Microshoots · Germination |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 04 Dec 2020 09:31 |
| Last Modified: | 29 Nov 2021 05:28 |
| URI: | http://ir.cftri.res.in/id/eprint/14705 |
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