A Triterpene Glycoside Fraction, TG from Gymnema sylvestre Ameliorates Insulin Resistance by Stimulating Glucose Uptake in 3T3L1 Adipocytes and C2C12 Skeletal Muscle Cells.

Rashmi, S. Shenoy and Manonmani, H. K. (2020) A Triterpene Glycoside Fraction, TG from Gymnema sylvestre Ameliorates Insulin Resistance by Stimulating Glucose Uptake in 3T3L1 Adipocytes and C2C12 Skeletal Muscle Cells. Journal of Biosciences and Medicines, 8. pp. 137-151.

PDF Journal of Biosciences and Medicines, 2020, 8, 137-151.pdf - Published Version Restricted to Registered users only Download (2MB)

Abstract

Type 2 diabetes mellitus (T2DM) which is characterized by insulin resistance in muscle and adipose tissues is a major problem worldwide. Plant based medications are well known from ancient times for possessing antidiabetic properties. Amongst them, Gymnema sylvestre (GS) is one such antidiabetic medicinal plant which has been used traditionally over the years for the treatment of T2DM. The aim of the present study was to investigate the effect of triterpene glycoside (TG), an active fraction isolated from ethanolic extract of Gymnema sylvestre (EEGS) on a battery of targets; glucose transporter (GLUT4), peroxisome proliferator activator receptor gamma (PPAR-γ), adiponectin and leptin involved in glucose transport and metabolism. No cytotoxic effects were observed in treated cells up to 600 µg/ml of TG as measured by MTT and ROS assays. Elevation of GLUT-4 and PPAR-γ by TG in association with glucose transport supported the upregulation of glucose uptake at concentrations of 50 and 100 µg/ml respectively. Additionally, TG showed higher expression of adiponectin and leptin, confirming the favorable pharmacological effect of TG on insulin resistance. The results were comparable to the known antidiabetic drug pioglitazone and commercial standard DAG. Thus TG could be considered as a safe nutraceutical candidate/functional phytoingredient in amelioration of insulin resistance.

Item Type:	Article
Uncontrolled Keywords:	Insulin Resistance, Gymnema sylvestre, GLUT-4, PPAR-γ, Glucose Uptake
Subjects:	600 Technology > 01 Medical sciences > 04 Diabetes Mellitus 600 Technology > 03 Agriculture > 04 Medicinal Plants
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Jan 2021 08:45
Last Modified:	01 Jan 2021 08:45
URI:	http://ir.cftri.res.in/id/eprint/14748

Actions (login required)

View Item