Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii

Dayananda, C. and Sarada, R. and Sila, Bhattacharya and Ravishankar, G. A. (2005) Effect of media and culture conditions on growth and hydrocarbon production by Botryococcus braunii. Process Biochemistry, 40. pp. 3125-3131.

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Abstract

The influence of the media constituents potassium nitrate, magnesium sulphate, dihydrogen potassium phosphate and ferric citrate on growth and hydrocarbon production in Botryococcus braunii (SAG 30.81) was investigated using response surface methodology (RSM). Among the individual variables, potassium nitrate and ferric citrate exhibited marked effects on the response functions (yield of biomass and hydrocarbon production). A high correlation coefficient (r ≥ 0.93, p ≤ 0.01) indicated the suitability of second-order polynomials to predict the response function. The optimum concentration of dihydrogen potassium phosphate, potassium nitrate, magnesium sulphate and ferric citrate were 0.16, 0.05, 0.10 and 0.025 g L-1, respectively, in the medium where hydrocarbon production was about 50% on dry weight basis. The optimum concentrations of dihydrogen potassium phosphate, potassium nitrate, magnesium sulphate and ferric citrate were found to be 0.0195, 0.05, 0.02 and 0.0185 g L�1, respectively, in the medium for 0.65 g L-1 biomass and 50.6% (w/w) hydrocarbon production. B.braunii (SAG 30.81) was identified as A race by mass spectra of hydrocarbons it produced.

Item Type:	Article
Uncontrolled Keywords:	Hydrocarbon production; Yield of biomass; Response surface methodology; Botryococcus braunii; Optimisation
Subjects:	600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
Divisions:	Grain Science and Technology Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Oct 2007 10:34
Last Modified:	11 May 2012 04:27
URI:	http://ir.cftri.res.in/id/eprint/1484

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