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Maternal high protein‑diet programs impairment of offspring’s bone mass through miR‑24‑1‑5p mediated targeting of SMAD5 in osteoblasts.
Govindraj, Ellur and Shinde, V. S. and Touseef Khan, Md. and Kunal, Sharan (2021) Maternal high protein‑diet programs impairment of offspring’s bone mass through miR‑24‑1‑5p mediated targeting of SMAD5 in osteoblasts. Cellular and Molecular Life Sciences, 78. pp. 1729-1744.
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Abstract
Maternal nutrition is crucial for the offspring’s skeleton development and the onset of osteoporosis later in life. While maternal low protein diet has been shown to regulate bone mass negatively, the effect of a high protein diet (HP) remains unexplored. Here, we found that C57BL/6 mice fed with HP delivered offspring with decreased skeletal mineralization at birth and reduced bone mass throughout their life due to a decline in their osteoblast maturation. A small RNA sequencing study revealed that miR-24-1-5p was highly upregulated in HP group osteoblasts. Target prediction and validation studies identified SMAD-5 as a direct target of miR-24-1-5p. Furthermore, mimic and inhibitor studies showed a negative correlation between miR-24-1-5p expression and osteoblast function. Moreover, ex vivo inhibition of miR-24-1-5p reversed the reduced maturation and SMAD-5 expression in the HP group osteoblasts. Together, we show that maternal HP diminishes the bone mass of the offspring through miR-24-1-5p.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Maternal nutrition · miRNA · Smad5 · Development · Bone · High protein diet |
| Subjects: | 600 Technology > 01 Medical sciences > 13 Nutrition-Human 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins |
| Divisions: | Molecular Nutrition |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 27 May 2021 06:52 |
| Last Modified: | 27 May 2021 06:52 |
| URI: | http://ir.cftri.res.in/id/eprint/14876 |
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