Adaptive evolution of engineered yeast for squalene production improvement and its genome-wide analysis.

Kalaivani, P. and Aneesha, A. and Nabarupa, Gupta and Sarma, Mutturi (2021) Adaptive evolution of engineered yeast for squalene production improvement and its genome-wide analysis. Yeast. pp. 1-14.

PDF Yeast. 2021;1–14.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

In the present study, the adaptive evolution of a metabolically engineered Saccharomyces cerevisiae strain in the presence of an enzyme inhibitor terbinafine for enhanced squalene accumulation via serial transfer leads to the development of robust strains. After adaptation for nearly 1500 h, a strain with higher squalene production efficiency was identified at a specific growth rate of 0.28 h−1 with a final squalene titer of 193 mg/L, which is 16.5-fold higher than the BY4741 and 3-fold higher over the metabolically engineered SK22 strain. Whole-genome sequencing comparison between the reference strain and the evolved variant SK23 has led to the identification of 462 single-nucleotide variants (SNVs) between both strains, with 102 SNVs affecting metabolism-related genes. It was also established that F420I mutation of ERG1 in S. cerevisiae improves squalene synthesis. Further, the effect of increased squalene on lipid droplet and neutral lipid pattern in the evolved mutant strains was investigated by fluorescent techniques proving that the neutral lipid content and clustering of lipid droplets increase with an increase in squalene.

Item Type:	Article
Uncontrolled Keywords:	adaptive evolution, lipid droplet, Nile red, Saccharomyces cerevisiae, squalene, whole-genome sequencing
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 24 Organic Chemistry 600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Jul 2021 05:55
Last Modified:	19 Jul 2021 05:55
URI:	http://ir.cftri.res.in/id/eprint/14898

Actions (login required)

View Item