Neoxanthin prevents H2O2‑induced cytotoxicity in HepG2 cells by activating endogenous antioxidant signals and suppressing apoptosis signals.

Deepika, Udayawara Rudresh and Tehreem, Maradagi and Nimish, Mol Stephen and Ayyadurai, Niraikulam and Kamini, Nambi Ramudu and Ganesan, P. (2021) Neoxanthin prevents H2O2‑induced cytotoxicity in HepG2 cells by activating endogenous antioxidant signals and suppressing apoptosis signals. Molecular Biology Reports, 48. pp. 6923-6934. ISSN 0301-4851

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Abstract

Background The liver has a solid inbuilt antioxidant defense system to regulate oxidative stress. However, exposure to an excessive level of ROS causes liver injury. This study examined the cytoprotective effect of neoxanthin, a xanthophyll antioxidant molecule isolated from Solanum trilobatum in stress-induced HepG2 cells. Methods and results The cytotoxic effect of H2O2 and cytoprotective potential of β-carotene, lutein, and neoxanthin was analyzed by WST-1 assay. The intracellular ROS level and mitochondrial membrane potential (MMP) were measured using DCFH-DA (2′, 7′-dichlorofluorescin diacetate) and JC-10 MMP assay. The expression of anti-oxidant and apoptotic markers was measured by western blot analysis. Neoxanthin pretreatment exhibited better protection than β-carotene and lutein against cell death caused by H2O2. It significantly arrested H2O2- mediated elevation of intracellular ROS levels and protected MMP. The intracellular antioxidant enzymes HO-1 and SOD-2 were upregulated by neoxanthin pretreatment. Neoxanthin also activated the protein expression of redox-sensitive transactivation factors, Nrf2 and NF-kB. The cytoprotective effect of neoxanthin was associated with increased expression of the anti-apoptotic protein, Bcl-2 and decreased pro-apoptotic protein Bax. Conclusions For the first time, our results demonstrate that neoxanthin offers adequate protection against stress-mediated cytotoxicity in hepatocytes by activating the intracellular antioxidant defense system and blocking apoptosis.

Item Type:	Article
Uncontrolled Keywords:	Carotenoids · Neoxanthin · HepG2 cells · Cytoprotection · Antioxidant enzymes · Apoptosis
Subjects:	600 Technology > 01 Medical sciences > 12 Metabolism
Divisions:	Molecular Nutrition
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Mar 2022 08:22
Last Modified:	29 Mar 2022 08:22
URI:	http://ir.cftri.res.in/id/eprint/15152

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