Preparation, characterization, radical scavenging property and antidiabetic potential of laminarioligosaccharides derived from laminarin.

Naveen, J. and Veeresh, T. and Gnanesh Kumar, B. S. and Sachin, R. Chaudhari and Baskaran, V. (2022) Preparation, characterization, radical scavenging property and antidiabetic potential of laminarioligosaccharides derived from laminarin. Algal Research, 63. p. 102642.

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Abstract

Laminarin is a polysaccharide (dietary fibre), rich in marine brown seaweed. Derived low molecular weight polysaccharides exhibit various biofunctional properties including antitumor, anti-apoptotic, anti-inflammatory, antiobese and antioxidant activity. Hence, the present study aimed to hydrolyse laminarin using 0.2 M Hydrochloric acid (HCl) into laminarioligosaccharides and their characterization and evaluation of antioxidant, antidiabetic (α-amylase and α-glucosidase inhibition) properties in vitro. Thin layer chromatography (TLC) revealed the hydrolysis of laminarin up to 7 laminarioligosaccharides, also the hydrolysis pattern was validated by ultraperformance liquid chromatography (UPLC) and 1H and 13C NMR. Mass spectrometry investigation confirmed the molecular mass of laminarioligosaccharides. 1H and 13C NMR revealed that the laminarioligosaccharides has both β(1–6)-linked and β(1–3)-linked glucose. Further, 2 h hydrolysate showed higher reductive capability (20 μg saccharide equivalent to 7.1 μg quercetin) and total antioxidant activity (20 μg saccharide equivalent to 6.7 μg vitamin C). The percent inhibition of 2 h hydrolysate was 42.9% (IC50-15.7 μg/mL) in 2,2-diphenyl-1-picrylhydrazyl- hydrate (DPPH) assay and 27.4% (IC50-28.4 μg/mL) in 2,2′ -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. Additionally, 2 h laminarioligosaccharides showed maximum inhibition of α-amylase (30 μg- 32.2% inhibition, IC50-46.6 μg/mL) and α-glucosidase (30 μg- 58.8% inhibition, IC50-25.5 μg/mL) activity. Thus, HCl hydrolysis of laminarin to laminarioligosaccharides could be a promising method for obtaining low molecular weight saccharides with enhanced biological activities.

Item Type:	Article
Uncontrolled Keywords:	Laminarin Hydrolysis Laminarioligosaccharides Antioxidant property Antidiabetic potential
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions:	Dept. of Biochemistry Meat Fish and Poultry Technology Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 May 2022 09:00
Last Modified:	09 May 2022 09:00
URI:	http://ir.cftri.res.in/id/eprint/15235

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