Lipidome analysis and characterization of Buglossoides arvensis acyltransferases that incorporate polyunsaturated fatty acids into triacylglycerols.

Prasad, Parchuri and Anjali, P. and Abdulrahman, Naeem and Timothy, P. Durrett and Ruth, Welti and Sreedhar, R. V. (2022) Lipidome analysis and characterization of Buglossoides arvensis acyltransferases that incorporate polyunsaturated fatty acids into triacylglycerols. Plant Science, 324. p. 111445.

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Abstract

Buglossoides arvensis is a burgeoning oilseed crop that contains an unique combination of ω-3 and ω-6 poly�unsaturated fatty acids (PUFA), constituting ~80–85% of seed triacylglycerols (TAGs). To uncover the critical TAG biosynthetic pathways contributing for high PUFA accumulation, we performed lipidome of developing seeds and characterized acyltransferases involved in the final step of TAG biosynthesis. During seed develop�ment, distribution of lipid molecular species in individual lipid classes showed distinct patterns from an early�stage (6 days after flowering (DAF)) to the middle-stage (12 and 18 DAF) of oil biosynthesis. PUFA-containing TAG species drastically increased from 6 to 12 DAF. The expression profiles of key triacylglycerol biosynthesis genes and patterns of phosphatidylcholine, diacylglycerol and triacylglycerol molecular species during seed development were used to predict the contribution of diacylglycerol acyltransferases (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferases (PDAT1 and PDAT2) to PUFA-rich TAG biosynthesis. Our analysis suggests that DGATs play a crucial role in enriching TAGs with PUFA compared to PDATs. This was further confirmed by fatty acid feeding studies in yeast expressing acyltransferases. BaDGAT2 preferentially incorpo�rated high amounts of PUFAs into TAG, compared to BaDGAT1. Our results provide insight into the molecular mechanisms of TAG accumulation in this plant and identify target genes for transgenic production of SDA in traditional oilseed crops.

Item Type:	Article
Uncontrolled Keywords:	Acyltransferases Buglossoides arvensis α-linolenic acid γ-linolenic acid Stearidonic acid Triacylglycerol
Subjects:	600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	13 Sep 2022 11:38
Last Modified:	13 Sep 2022 11:38
URI:	http://ir.cftri.res.in/id/eprint/15755

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