Antimicrobial compound from microorganism of marine origin and elucidation of their characteristics.

Lidiya, C. Johny (2022) Antimicrobial compound from microorganism of marine origin and elucidation of their characteristics. Doctoral thesis, Central Food Technological Research Institute.

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Abstract

Antimicrobial peptides (AMPs) are effective antimicrobial agents found in plants, animals and microorganisms. The present work focuses on AMPs from marine�derived bacteria and their potential to control foodborne pathogens. We have screened and isolated AMP producing bacteria (5 nos.) from the West coast region of South India. The Box-Behnken design was applied to optimize the process variables for maximum production of antibacterial compounds by the selected bacterial isolates. After optimization, the two cultures (FTL6, FTL7) with a comparatively high fold increase in AMP production were selected for further studies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that bacterial isolate FTL6 is closely related to B. subtilis, and FTL7 is closely related to B. velezensis. Purification of AMP from FTL6 and FTL7 was carried out and characterized. Through genome annotation, the presence of genes responsible for the synthesis of AMP produced by FTL7 was identified. Further, in silico studies supported the efficiency of these AMPs as antibacterial agents in the food industry.

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Antimicrobial peptides, marine origin, foodborne pathogens,
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 06 Marine Microbiology 600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions:	Meat Fish and Poultry Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 May 2023 04:55
Last Modified:	19 May 2023 04:55
URI:	http://ir.cftri.res.in/id/eprint/16415

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