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Infuence of vitamin E on the cellular uptake and transport of selenium from wheat and pearl millet across Caco‑2 cell monolayer.
Anjum, Khanam and Dhanya, K. and Kalpana, Platel (2023) Infuence of vitamin E on the cellular uptake and transport of selenium from wheat and pearl millet across Caco‑2 cell monolayer. Journal of Food Measurement and Characterization, 17. pp. 1480-1491.
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Abstract
The purpose of the current study was to evaluate the efect of exogenous addition of vitamin E on the cellular uptake and transport of selenium (Se) across Caco-2 cell monolayer from wheat and pearl millet. Cellular bioavailability of Se was also determined by evaluating the activity of Se dependent enzymes such as glutathione peroxidase (GPx) and thioredoxin reductase (TR). Transport and uptake of Se was by passive difusion (transcellular transport), which was time dependent. Uptake of selenomethionine (SeMet) and selenocysteine (SeCys2) by the Caco-2 cells incubated with wheat and pearl mil�let was signifcantly enhanced (p<0.001) in the presence of vitamin E, which, however, was signifcantly decreased by heat processing (p<0.001). The mRNA expression of the genes encoding the two Se dependant enzymes, GPx and TR was signifcantly upregulated in wheat and pearl millet respectively, upon the addition of vitamin E. The extent of upregulation ranged from 80 to 100%.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Caco-2 cells · Glutathione peroxidase · Thioredoxin reductase |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 17 Vitamin Biochemistry 600 Technology > 08 Food technology > 21 Cereals > 04 Wheat 600 Technology > 08 Food technology > 21 Cereals > 05 Ragi (Finger Millet) |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 24 May 2023 10:53 |
| Last Modified: | 24 May 2023 10:53 |
| URI: | http://ir.cftri.res.in/id/eprint/16457 |
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