Vanillic acid potentiates insulin secretion and prevents pancreatic β‑cells cytotoxicity under H2O2‑induced oxidative stress.

Chandan, Muddahally Naganna and Yogendra Prasad, K. and Mahendra, V. P. and Ganesan, P. and Ravi, Kumar (2023) Vanillic acid potentiates insulin secretion and prevents pancreatic β‑cells cytotoxicity under H2O2‑induced oxidative stress. Molecular Biology Reports, 50. pp. 1311-1320. ISSN 0301-4851

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Abstract

Background Oxidative stress is known to impair cellular functions and, therefore, plays a signifcant role in the pathophysi�ology of various diseases, including diabetes. The persistently elevated glucose levels may cause enhanced mitochondrial reactive oxygen species generation, which in turn can damage the pancreatic β-cells. In this study, we have investigated the efect of vanillic acid on preventing H2O2-induced β-cells death and retaining its insulin secretion potentiating efect in the presence of H2O2. Methods The insulin secretion from the BRIN-BD11 cells was quantifed using ELISA-based assays. The viability of the cells was assessed by estimated by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) colorimet�ric assay and DAPI staining. The expression levels of apoptotic and antioxidant proteins were estimated by western blot experiments. Results Vanillic acid protected pancreatic β-cells viability and function under the H2O2 oxidative stress condition. The Erk1/2 activation appears to play an important role in vanillic acid potentiated insulin secretion and protection of the β-cells in the presence of H2O2. Vanillic acid pretreated cells exhibited enhanced expression of antioxidant enzymes such as catalase and SOD-2 and reduced the expression of proapoptotic markers such as BAX and BAD. In addition, it also enhanced the expres�sion of oxidative stress-sensitive transcription factor Nrf-2 and cell survival protein Akt. Conclusion The present study shows that vanillic acid potentiates insulin secretion and protects pancreatic β-cells from H2O2-induced oxidative stress.

Item Type:	Article
Uncontrolled Keywords:	Vanillic acid · Oxidative stress · H2O2 · ROS · Pancreatic β-cells
Subjects:	600 Technology > 01 Medical sciences > 04 Diabetes Mellitus 600 Technology > 08 Food technology > 15 Flavour/Fragrance/Perfumes
Divisions:	Molecular Nutrition
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 May 2023 04:33
Last Modified:	26 May 2023 04:33
URI:	http://ir.cftri.res.in/id/eprint/16474

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