Nanoencapsulation of apocynin and vanillic acid extracted from Picrorhiza kurroa Royle ex Benth plant roots and its characterisation

Vallamkondu, Manasa and Sivathasan, Shubangi and Rakshitha Rame, Gowda and Anitta, Jose and Muthukumar, S. P. and Krupashree, Krishnaswamy (2024) Nanoencapsulation of apocynin and vanillic acid extracted from Picrorhiza kurroa Royle ex Benth plant roots and its characterisation. Heliyon, 10. pp. 1-13. (Submitted)

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Abstract

Picrorhiza kurroa Royle ex Benth (P. kurroa) is an important medicinal plant in the ayurvedic system for treating various liver and inflammatory conditions. The present study aimed to extract the phytocompounds from various extracts (Acetone, Chloroform, Ethanol, Ethyl acetate, Hexane, and Methanol) of P. kurroa. Further, the major phytocompounds were nano-encapsulated by PLGA (Poly-lactic-co-glycolic acid) method and characterized to enhance activity towards the target. The highest polyphenolic value was found to be 323.2 ± 16.6 and 316.3 ± 12.1 μg GAEq./mg in ethanolic and methanolic extracts. The highest flavonoid value was found to be 280.3 ± 19.8 and 300.8 ± 15.2 in ethanolic and methanolic extracts μg QEq./mg. P. kurroa exhibited DPPH radical scavenging with IC50 of 38.2 ± 1.1 and 43.7 ± 1.8 μg/mL and also showed potent ferric reducing power and total antioxidant activities. The major phytocompounds, such as apocynin (AP) and vanillic acid (VA), were confirmed using HPLC. Further, the nano-encapsulation of apocynin and vanillic acid successfully achieved by PLGA methods. The average particle size of nanoencapsulated apocynin, vanillic acid is 350 nm, 204.4 nm, and zeta potential were

Item Type:	Article
Uncontrolled Keywords:	Nanoencapsulation, vanillic acid, Picrorhiza kurroa Royle
Subjects:	500 Natural Sciences and Mathematics > 10 Plants
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	22 Apr 2024 09:37
Last Modified:	22 Apr 2024 09:37
URI:	http://ir.cftri.res.in/id/eprint/17502

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