Gallic acid inhibits Staphylococcus aureus RecA protein functions: Role in countering antibiotic resistance in bacteria.

Kajal, Kiran and Neelakanteshwar Patil, K. (2024) Gallic acid inhibits Staphylococcus aureus RecA protein functions: Role in countering antibiotic resistance in bacteria. Journal of Applied Microbiology, 135. pp. 1-11.

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Abstract

Aim: R ecombinase R ecA and its homologs pla y a k e y role in homologous recombination DNA repair and re viv e stalled replication fork DNA synthesis. R ecA pla y s an essential role in the e v olution of antibiotic-resistant strains via stress-induced DNA repair mechanisms during the SOS response. A ccordingly, R ecA has become an attractive target to slow down antibiotic resistance rates and prevent mutations in pathogenic bacterial species. Methods and results: We emplo y ed R ecA conserv ed activities: DNA binding, displacement loop formation, strand e x change, ATP h y droly sis, and L e xA clea v age, to elucidate the inhibitory role of gallic acid on Staphylococcus aureus RecA functions. Gallic acid inhibition of the SOS response by western blot analysis and its antibacterial activity were measured. The gallic acid inhibited all the canonical activities of S. aureus RecA protein. Conclusion: The natural phenolic compound gallic acid interferes with RecA protein DNA complex formation and inhibits activities such as displacementloop formation, strand exchange reaction, ATP hydrolysis, and coprotease activity of S. aureus . Additionally, gallic acid can obstruct ciproflo xacin-induced R ecA e xpression and e v entually confer the inhibitor y role of gallic acid in the SOS sur vival mechanism in S. aureus. Impact Statement These findings indicate that gallic acid acts as a RecA inhibitor. The strategy of disrupting the vital processes regulated by the RecA protein by natural chemicals might be a promising target for their use in drug-resistant and drug-susceptible foodborne bacterial strains. They might possibly be recommended as adju v ants along with existing antibiotics, which might enhance the effect of existing antibiotics and slow down the emergence of antibiotic resistance in microorganisms

Item Type:	Article
Uncontrolled Keywords:	Staphylococcus aureus ; recombination; antibiotic resistance; gallic acid; RecA; DNA repair
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Dec 2024 09:20
Last Modified:	27 Dec 2024 09:20
URI:	http://ir.cftri.res.in/id/eprint/18899

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