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Production of Antioxidant, Angiotensin‑Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder
Balaji Wamanrao, Kanwate and Kalpana, Patel and Sandesh Suresh, Karkal and Deependra, Rajoriya and Kunal, Sharan and Tanaji Kudre, G. (2024) Production of Antioxidant, Angiotensin‑Converting Enzyme Inhibitory and Osteogenic Gelatin Hydrolysate from Labeo rohita Swim Bladder. Marine Biotechnology, 26. pp. 404-420.
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Abstract
Optimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-3- ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydrolysis temperature, and 94-min hydrolysis time. The resulting SBGH obtained at 19.92% DH exhibited the DPPH (24.28 µM TE/mg protein), ABTS (34.47 µM TE/mg protein), TAO (12.01 µg AAE/mg protein), and ACE inhibitory (4.91 µg/mg protein) activity. Furthermore, SBGH at 100 µg/ml displayed osteogenic property without any toxic effects on MC3T3-E1 cells. Besides, the protein content of rohu swim bladder gelatin (SBG) and SBGH was 93.68% and 94.98%, respectively. Both SBG and SBGH were rich in glycine, proline, glutamic acid, alanine, arginine, and hydroxyproline amino acids. Therefore, SBGH could be an effective nutraceutical in functional food development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Rohu swim bladder, Gelatin hydrolysate, Response surface methodology, Antioxidant activity, ACE inhibitory activity, Osteogenic property |
| Subjects: | 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish 600 Technology > 08 Food technology > 32 Antioxidants |
| Divisions: | Meat Fish and Poultry Technology |
| Depositing User: | Somashekar K S |
| Date Deposited: | 20 Jan 2025 06:54 |
| Last Modified: | 20 Jan 2025 06:54 |
| URI: | http://ir.cftri.res.in/id/eprint/18929 |
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