Carissa spinarum seed oil: a newer perspective on dietary lipids and nutraceuticals – phytochemical characterization, Lipidomics, and pre-toxicological assessment

Manoj, Prabhakaran and Moorthy Karthika, Selvi and Panneerselvam, Vijayaraj and Nandini Prasad, Shetty and Sreerama, Y. N. (2024) Carissa spinarum seed oil: a newer perspective on dietary lipids and nutraceuticals – phytochemical characterization, Lipidomics, and pre-toxicological assessment. Journal of Food Measurement and Characterization, 18. pp. 4177-4191.

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Abstract

Carissa spinarum (CS) is a wild edible fruit crop with medicinal properties, but information about Carissa spinarum Seed Oil (CSSO) is limited, particularly regarding its phytochemicals and lipidomes. This study aims to bridge this knowledge gap by providing comprehensive data on CSSO’s physicochemical properties, nutraceutical content, and antioxidant potential, exploring its potential applications. The CSSO exhibited approximately 8.2 to 9% oil content, with a major proportion of monounsaturated fatty acids (MUFA) at 66.24%. Gas chromatography-mass spectrometry (GC/MS) analysis reveals that oleic acid content was 65.2%, followed by palmitic acid (14.59%), stearic acid (8.96%), linoleic acid (8.9%), and linolenic acid (1.94%) in the Oil. The HRMS analysis of the TAG molecular species distribution confirmed that triolein (C54:3) was the predominant triacylglycerol in CSSO. Nutraceutical profiling revealed the presence of tocols (32 mg), phytosterols (1486.33 mg), and squalene (74.18 mg) per 100 g of CSSO, displaying strong antioxidant activity. FTIR, DSC, and HRMS analysis confirmed the characteristics of CSSO. Additionally, CSSO cake contained 10.12% protein and various minerals, making it a potential dietary protein source. The fatty acid composition and physicochemical parameters of CSSO enhance its acceptability for human consumption. Additionally, CSSO contains beneficial compounds such as β-Sitosterol and α-tocopherol, contributing to its antioxidant and health-promoting properties. Further, the in vitro cytotoxicity study ensures that it is safe and non-toxic to normal cells. This study provides the first comprehensive

Item Type:	Article
Uncontrolled Keywords:	Carissa spinarum seed oil, Oil cake, Fatty acid profile, TAG molecular species, Lipidome profile Tocopherols · Toxcity · HRMS
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry 600 Technology > 01 Medical sciences > 17 Toxicology 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions:	Plant Cell Biotechnology
Depositing User:	Somashekar K S
Date Deposited:	20 Jan 2025 06:51
Last Modified:	20 Jan 2025 06:51
URI:	http://ir.cftri.res.in/id/eprint/18941

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