Physicochemical characteristics of dietary fiber polysaccharides extracted from Murraya koenigii leaves and their functional role on gut homeostasis

Priya, Mondal and Sumathy, Ramasamy and Rajendran, Amalraj and Claretta Jasmine, Anthonira and Subramaniyan, Gokila and Syed Musthapa, Meeran (2025) Physicochemical characteristics of dietary fiber polysaccharides extracted from Murraya koenigii leaves and their functional role on gut homeostasis. International Journal of Biological Macromolecules (293). pp. 1-22.

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Abstract

Dietary fiber (DF) is an indigestible carbohydrate in plant foods that supports various physiological functions. This study aimed to extract the soluble and insoluble dietary fiber (DF) from the curry leaves and investigate their physicochemical properties as well as their functional role in the homeostasis of the gut microbiome. The study observed that insoluble-DF (IDF) yielded higher amounts than soluble-DF (SDF) across alkali, acid, and water extraction methods. Acid-extracted SDF showed the highest polysaccharide content (91.58 ± 1.53 %). Among IDFs, alkali-extracted IDF showed the highest polysaccharide content (81.93 ± 1.94 %). Glucose, arab­inose, and xylose were identified as major monosaccharides. IDF had a larger particle size (463.5 ± 14.2 μm)compared to SDF (1.23 ± 15.55 μm), which influenced its physicochemical properties. IDF displayed better oil-holding capacity, while SDF showed superior water-holding capacity, potentially impacting glucose diffusion and cholesterol micelle formation. Furthermore, SDF and IDF promoted the growth of beneficial gut bacteria and increased production of short-chain fatty acids, which play critical roles in physiological regulation. Acid-extracted SDF restored gut homeostasis by increasing species richness and diversity, predominantly increasing beneficial bacteria and reducing pathogenic bacteria in LPS-induced dysbiotic mice. This study reveals the impact of extraction methods on the physicochemical and functional properties of curry leaves-DF, underscoring its promise as a functional food for gut health.

Item Type:	Article
Uncontrolled Keywords:	Curry leaves, Dietary polysaccharides, Soluble dietary fiber Insoluble dietary fiber, Gut microbiota, Gut metabolites Dysbiosis
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry 600 Technology > 08 Food technology > 18 Processed foods > 01 Dietary Fiber 600 Technology > 08 Food technology > 30 Spices/Condiments > 06 Curry Leaf
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	25 Feb 2025 04:35
Last Modified:	25 Feb 2025 04:35
URI:	http://ir.cftri.res.in/id/eprint/19138

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