Protective effect of diindolylmethane-enriched dietary cabbage against doxorubicin-induced cardiotoxicity in mice

Jagadish, Natesh and Priya, Mondal and Devendra Jaganath, Haware and Dhanamjai, Penta and Syed Musthapa, Meeran (2024) Protective effect of diindolylmethane-enriched dietary cabbage against doxorubicin-induced cardiotoxicity in mice. Journal of Applied Toxicology. pp. 874-891.

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Abstract

Chemotherapy with doxorubicin (Dox) can lead to cardiotoxic effects, presenting a major complication in cancer therapy. Diindolylmethane (DIM), derived from cruciferous vegetables like cabbage, exhibits numerous health benefits. However, its clinical application is limited because of low bioavailability and suboptimal natural concentra- tions in dietary sources. To address this limitation, we developed a processing meth-odology, specifically fermentation and boiling, to enhance DIM levels in cabbage. High-performance liquid chromatography (HPLC) analysis revealed a threefold DIM increase in fermented cabbage and a substantial ninefold increase in fermented- boiled cabbage compared to raw cabbage. To evaluate the clinical implications, we formulated a DIM-enriched diet and administered it to mice undergoing Dox treat- ment. Our in vivo results revealed that Dox treatment led to cardiotoxicity, mani- fested by changes in body and heart weight, increased mortality, and severe myocardial tissue degeneration. Dietary administration of the DIM-enriched diet enhanced antioxidant defenses and inhibited apoptosis in the cardiac tissue by inter- fering with mitoptosis and increasing antioxidant enzyme expression. Interestingly, we found that the DIM-enriched diet inhibited the nuclear translocation of NF-kB in cardiac tissue, thereby downregulating the expression of inflammatory mediators such as TNF-α and IL-6. Further, the DIM-enriched diet significantly reduced serum cardiac injury markers elevated by Dox treatment. These results suggest that the DIM-enriched cabbage diet can serve as a complementary dietary intervention for cancer patients undergoing chemotherapy. Further, our research highlights the role of plant-based diets in reducing treatment side effects and improving the quality of life for cancer patients.

Item Type:	Article
Uncontrolled Keywords:	cabbage, cardiotoxicity, cruciferous vegetables, diindolylmethane, doxorubicin
Subjects:	600 Technology > 08 Food technology > 18 Processed foods > 01 Dietary Fiber 600 Technology > 08 Food technology > 23 Vegetables
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	07 Mar 2025 05:31
Last Modified:	07 Mar 2025 05:31
URI:	http://ir.cftri.res.in/id/eprint/19224

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