Syringic acid exerts antiangiogenic activity by downregulation of VEGF in zebraﬁsh embryos

Gowri, Karthik and Mangalagowri, Angappan and Arunkumar, VijayaKumar and Sukumaran, Natarajapillai (2014) Syringic acid exerts antiangiogenic activity by downregulation of VEGF in zebraﬁsh embryos. Biomedicine & Preventive Nutrition, 4. pp. 203-208.

PDF
1-s2.0-S2210523914000087-main.pdf - Published Version
Restricted to Registered users only
Download (1MB) | Request a copy

Abstract

Angiogenesis, the formation of new blood vessels is essential for the conservancy of normal cell func- tions. The dysfunction and/or increase in angiogenesis may lead to uncontrolled cell proliferation of cells resulting in cancer. Inhibiting angiogenic factors using natural compound has substantial hope in cancer research. Hence, we investigated syringic acid (SA), a naturally occurring phenolic acid for its antiangio- genic property using the zebraﬁsh embryo in vivo model. Morphological observations of the SA treated embryos were analyzed to evaluate the toxicity of the compound. RBC staining was performed to evaluate the ISV inhibition in zebraﬁsh embryos. Further, to determine the mechanism behind ISV inhibition, a real-time reverse-transcriptase polymerase chain reaction and western blot analysis was done to evaluate VEGF mRNA and protein expression respectively. Our result showed that SA didn’t affect the morphology up to 50␮M. The apparent angiogenic effect was seen from 20 to 50 ␮M, which signiﬁcantly reduced number of circulating red blood cells in ISV region compared to that of control. At 50 ␮M there was no visible RBC present in the ISV region of the embryos. Further, Real-time PCR and western blot analysis showed, SA inhibited VEGF mRNA expression and protein expression respectively in a dose dependent manner. These ﬁndings altogether suggest that syringic acid may have antiangiogenic activity by down regulating VEGF mediated pathway thereby having potential therapeutic beneﬁt and promises to be a weapon against cancer.

Item Type:	Article
Uncontrolled Keywords:	Antiangiogenesis, Syringic acid, VEGF, Zebraﬁsh
Subjects:	600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
Divisions:	Dept. of Lipid Science
Depositing User:	Somashekar K S
Date Deposited:	11 Mar 2025 08:49
Last Modified:	11 Mar 2025 08:49
URI:	http://ir.cftri.res.in/id/eprint/19272

Actions (login required)

View Item