Synbiotic pineapple beverage increases life span in Caenorhabditis elegans, ameliorates cognitive impairment, and restores gut microbiome diversity in D‑galactose‑induced aged C57BL/6 mice

Neelam Anil, Revankar and Siddaraju, Anusha and Serva Peddha, Muthukumar and Pradeep Singh, Negi (2025) Synbiotic pineapple beverage increases life span in Caenorhabditis elegans, ameliorates cognitive impairment, and restores gut microbiome diversity in D‑galactose‑induced aged C57BL/6 mice. Journal of Biogerontology. pp. 26-99.

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Abstract

The incidence of age-associated ailments has increased proportionately with the expansion of the aging demographic. This study aimed to evaluate the anti-aging potential of synbiotic pineapple bever- age formulated with 100% pineapple juice, 1% inulin, and Lacticaseibacillus rhamnosus ATCC 53103 (10 log CFU) in Caenorhabditis elegans and D-galactose age-induced mice. The synbiotic juice-treated nema- todes exhibited a 24.52% increase in their lifespan, accompanied by lower levels of reactive oxygen spe- cies and improved structural functions. In vivo stud- ies demonstrated that synbiotic treatment positively influences age-induced mice’s cerebellar function and spatial memory. Additionally, the synbiotic bev- erage containing 8–10 log CFU of Lacticaseibacillus rhamnosus showed a protective effect against hip- pocampal neuron damage. The control group dis- played a higher Firmicutes/Bacteroides (F/B) ratio, whereas the significantly lower F/B ratio in the dis- eased groups indicated a reversal of microbial imbal- ance caused by D-galactose exposure. Furthermore, the consumption of synbiotic beverage mitigated tel- omere shortening in aged mice. The results highlight the anti-aging effects of a pineapple beverage formu- lated with Lacticaseibacillus rhamnosus and inulin as a synbiotic intervention. This study suggests that die- tary interventions incorporating prebiotics and probi- otics may serve as promising strategy for combating age-related disorders and promoting healthy aging.

Item Type:	Article
Uncontrolled Keywords:	Inulin · Lacticaseibacillus rhamnosus · Pineapple · Synbiotics · Caenorhabditis elegans · D-galactose-induced aged mice
Subjects:	600 Technology > 07 Beverage Technology 600 Technology > 08 Food technology > 24 Fruits
Divisions:	Fruit and Vegetable Technology Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	25 Jun 2025 06:01
Last Modified:	25 Jun 2025 06:01
URI:	http://ir.cftri.res.in/id/eprint/19594

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