Novel Probiotic Strains From Honey and Fermented Edible Flowers Mitigate Chronic Cortisol-Induced Leaky Gut Syndrome in the CaCo 2 Cell Model

Neelam Anil, Revankar and Pradeep Singh, Negi (2025) Novel Probiotic Strains From Honey and Fermented Edible Flowers Mitigate Chronic Cortisol-Induced Leaky Gut Syndrome in the CaCo 2 Cell Model. Journal of Food Science, 90. pp. 1-19.

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Abstract

Chronic stress and elevated cortisol levels can compromise intestinal barrier function, potentially leading to leaky gut syndrome and associated health conditions. The probiotic potential of novel strains isolated from honey and edible flowers was evaluated in the present study. Five strains identified as Lactiplantibacillus plantarum (MP), Enterococcus lactis (PERI), Lactiplantibacillus plantarum (PARI), Lactocasiebacillus rhamnosus (M1A), and Enterococcus nangengnisis (KK) exhibited strong bile salt hydrolase (BSH) activity. Furthermore, these strains produced short-chain fatty acids, including acetic acid (8.0 to 10.0 mM), butyric acid (0.3 to 0.5 mM) and propionic acid (1.8 to 2.5 mM), known to support gut health and immune modulation. Under chronic cortisol exposure, L. rhamnosus (M1A), L. plantarum (BAN), and L. plantarum (PARI) significantly alleviated tight junction disruption, experimentally proven by FITC dextran leakage and gene expression of zona occludin-1 (≤3-fold increase) and cadherin (≤4.36-fold increase) compared to the cortisol-treated CaCo 2 cell model. These findings indicate that the novel probiotic strains can enhance intestinal barrier integrity under chronic stress conditions in a model system; however, they warrant further investigation for their potential application in functional foods targeting gut health.

Item Type:	Article
Uncontrolled Keywords:	chronic cortisol stress, lactic acid bacteria, leaky gut, short-chain fatty acids
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology 600 Technology > 08 Food technology > 25 Sugar/Starch/Confectionery
Divisions:	Fruit and Vegetable Technology
Depositing User:	Somashekar K S
Date Deposited:	25 Sep 2025 09:51
Last Modified:	25 Sep 2025 09:51
URI:	http://ir.cftri.res.in/id/eprint/19879

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