Unraveling the Photoregulatory Mechanisms of Capsaicinoids Biosynthesis and Accumulation of Capsaicinoids in Capsicum annuum In Vitro Cultures

Monisha, Arya and Gyanendra, Kumar and Parvatam, Giridhar (2025) Unraveling the Photoregulatory Mechanisms of Capsaicinoids Biosynthesis and Accumulation of Capsaicinoids in Capsicum annuum In Vitro Cultures. Physiologia Plantarum, 177. pp. 1-11.

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Abstract

Light is one of the essential environmental factors that significantly influences the biosynthesis of secondary metabolites in plants. To explicate their effect, exposure of monochromatic LED light spectra (yellow, blue, green, red, and white) on the accu- mulation of capsaicinoids, phenolic compounds, antioxidant capacity, and regulation of transcriptional changes in capsaicinoids biosynthesis genes at 24, 48, and 72 h in chili callus cultures was investigated. Blue light significantly increased capsaicin and dihydrocapsaicin contents by 41.43-fold and 4.36-fold, respectively, compared to white light. Pearson correlation analysis con- firmed a strong positive correlation between total phenolics, total flavonoids, and antioxidant capacity (r > 0.85). Gene expression analysis revealed that the biosynthetic genes (pAMT, KAS, ACS, ACL, CL, C4H, and CS) were upregulated, and their associated transcription factors—MYB, ERF, and JERF—showed varied expression, indicating their putative regulatory role in capsaici- noid biosynthesis under different monochromatic LED conditions. Our findings demonstrate that the targeted monochromatic LED lights can enhance bioactive compound production in C. annuum callus cultures, providing a favorable strategy for feasible production of the industrially important compound “capsaicin” on a large scale for pharmaceutical and food applications.

Item Type:	Article
Uncontrolled Keywords:	antioxidant capacity, capsaicinoids, chili callus cultures, monochromatic LED light, transcription factor
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Plant Cell Biotechnology
Depositing User:	Somashekar K S
Date Deposited:	07 Oct 2025 06:05
Last Modified:	07 Oct 2025 06:05
URI:	http://ir.cftri.res.in/id/eprint/19884

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