Functional implications of arginine-121 in RuvA oligomerisation and RuvAB-mediated branch migration in the Gram-positive Listeria monocytogenes

Deeksha, Sugunan and Bianco Piero, R. and Neelakanteshwar Patil, K. (2025) Functional implications of arginine-121 in RuvA oligomerisation and RuvAB-mediated branch migration in the Gram-positive Listeria monocytogenes. Federation of European Biochemical Societies. pp. 1-18.

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Abstract

In prokaryotes, the RuvAB complex drives Holliday junction (HJ) branch migration, but the relative importance of RuvA tetramers versus octamers remains debatable and unexplored in Gram-positive bacteria. In this study, we aimed to determine whether RuvA from Listeria monocytogenes (LmRuvA) is active as a tetramer or octamer in branch migration. We identi- fied arginine-121 as being critical for the formation of the tetramer–tetramer interface. Mutation of arginine-121 to aspartate results in a protein that exists in a dimer to tetramer equilibrium in solution (unlike other octamer- deficient mutants from earlier studies), binds to the HJ as a tetramer only, interacts poorly with RuvB, and cannot catalyse branch migration. Collec- tively, these findings suggest that the ability of LmRuvA to bind HJs as an octamer is critical to branch migration.

Item Type:	Article
Uncontrolled Keywords:	branch migration; Holliday junction; homologous recombination; Listeria monocytogenes; RuvAB proteins
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Food Microbiology
Depositing User:	Somashekar K S
Date Deposited:	21 Nov 2025 06:21
Last Modified:	21 Nov 2025 06:21
URI:	http://ir.cftri.res.in/id/eprint/20097

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