Discrimination of raffinose and planteose based on porous graphitic carbon chromatography in combination with mass spectrometry

Lijina, P. and Gnanesh Kumar, B. S. (2023) Discrimination of raffinose and planteose based on porous graphitic carbon chromatography in combination with mass spectrometry. Journal of Chromatography B, 1224. pp. 1-6.

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Abstract

Raffinose and planteose are non-reducing, isomeric trisaccharides present in many higher plants. Structurally, they differ in the linkage of α-D-galactopyranosyl to either glucose C(6) or to C (6′ ) of fructose, respectively and thus differentiating each other is very challenging. The negative ion mode mass spectrometric analysis is shown to distinguish planteose and raffinose. However, to facilitate the robust identification of planteose in complex mixtures, herein, we have demonstrated the use of porous graphitic carbon (PGC) chromatography combined with QTOF-MS2 analysis. The separation of planteose and raffinose was achieved on PGC, wherein both have recorded different retention time. Detection through MS2 analysis revealed the specific fragmentation patterns for planteose and raffinose that are distinctive to each other. The applicability of this method on oligosaccharides pool extracted from different seeds showed clear separation of planteose that allowed unambiguous identifica- tion from complex mixtures. Therefore, we propose PGC-LC-MS/MS can be employed for sensitive, throughput screening of planteose from wider plant sources.

Item Type:	Article
Uncontrolled Keywords:	Planteose, Raffinose, Porous graphitic carbon, Negative mode Mass spectrometry
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	19 Dec 2025 05:02
Last Modified:	19 Dec 2025 05:02
URI:	http://ir.cftri.res.in/id/eprint/20188

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